PI-110

K. Delano, E. Pope, B. Kapur, G. Koren; Hospital for Sick Children, Toronto, ON, Canada

BACKGROUND: This is the first national study worldwide that objectively measures the rate of heavy alcohol consumption during pregnancy. Meconium analysis of the established biomarker of in utero alcohol exposure, fatty acid ethyl esters (FAEEs) is used as an objective measure of heavy alcohol exposure.
METHODS: In collaboration with the MIREC study, 2,000 pregnant women were recruited across Canada, with 1,537 consenting to meconium analysis. Meconium samples were analyzed for 4 FAEEs via GC-MS analysis. A positive sample has a total [FAEE] greater than 2 nmol/g. Alcohol self-report was collected via questionnaire and compared to analytical results. The rate of false-positive results increases in a predictable manner with delayed sample collection and is accounted for in incidence rate calculation.
RESULTS: Thus far, 690 samples have been processed with 609 eligible for analysis. Analysis results with maternal alcohol consumption self-report data are summarized in the table. The incidence rate of heavy fetal alcohol exposure in Canada was 2.55%.
CONCLUSION: This study is the first of its kind to objectively measure the rate of heavy alcohol consumption during pregnancy in Canada. Because 40% of heavily exposed fetuses develop FASD, our study predicts a rate of at least 1% of FASD in Canada, corresponding to 3,750 new cases yearly.
FAEE Analysis ResultMeconium Sample Collection and Alcohol Self-Report
(No drinking (ND), Social drinking (SD), Above social drinking (AD))
Total
< 24 hours
(ND, SD, AD)
24 - 48 hours
(ND, SD, AD)
> 48 hours
(ND, SD, AD)
N/A
(ND, SD, AD)
Positive2
(2, 0, 0)
15
(12, 2, 1)
12
(10, 2, 0)
5
(4, 1, 0)
34
Negative54
(45, 7, 2)
209
(164, 29, 16)
242
(197, 26, 19)
10
(7, 1, 2)
515
Inconclusive
(Transitional meconium to stool)
6
(6, 0, 0)
19
(15, 2, 2)
33
(28, 3, 2)
2
(2, 0, 0)
60